Spaces:
Running
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CPU Upgrade
Running
on
CPU Upgrade
Andrew Stirn
commited on
Commit
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f373c96
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Parent(s):
b054645
small type
Browse files
tiger.md
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@@ -21,7 +21,7 @@ The tool supports two methods for transcript entry:
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The tool has three run modes:
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1) Report all on-target gRNAs for each provided transcript.
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2) Report the top 10 most active, on-target gRNAs for each provided transcript. This mode allows for the optional identification of off-target effects. For off-target avoidance, please note that higher
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3) Report the top 10 most active, on-target gRNAs for each provided transcript and their titration candidates (all possible single mismatches). A higher `Guide Score` (closer to 1) corresponds to greater transcript knockdown.
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The tool uses Gencode v19 (protein-coding and non-coding RNAs) to identify potential off-target transcripts.
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The tool has three run modes:
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1) Report all on-target gRNAs for each provided transcript.
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2) Report the top 10 most active, on-target gRNAs for each provided transcript. This mode allows for the optional identification of off-target effects. For off-target avoidance, please note that a higher `Guide Score` (closer to 1) corresponds to *more* likely off-target effects.
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3) Report the top 10 most active, on-target gRNAs for each provided transcript and their titration candidates (all possible single mismatches). A higher `Guide Score` (closer to 1) corresponds to greater transcript knockdown.
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The tool uses Gencode v19 (protein-coding and non-coding RNAs) to identify potential off-target transcripts.
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